Release and labelling of sialic acids with 1,2-diamino-4,5-methylenedioxybenzene.2HCl (DMB) for fluorophore detection of chromatographically separated sialic acid variants. Kit sufficient for 96 samples (plus some dead volume for liquid handling robot use).
The LudgerTag DMB Sialic Acid labelling Kit has been developed for the qualitative analysis of sialic acids.
The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction.