Analysis by HPLC of sialic acid variants fluorophore labelled with DMB (1,2-diamino-4,5-methylenedioxybenzene.2HCl) using the LudgerTag DMB Sialic Acid labelling Kit.
Two LudgerSep R1 columns set up in series can be used for analysis of 2AB labelled antibody glycans. This is particularly good for separation of G1, G2, G3 glycan, fucosylated/nonfucosylated glycan separation, bisecting GlcNAc/non-bisecting GlcNAc glycan separation and for separation of MAN5 from neutral complex biantennary glycans.