LudgerClean protein-binding membrane plate for unlabelled glycan enrichment

Each pack contains one hydrophobic PVDF 96-well plate. For removal of enzymes and other proteins from unlabelled glycan mixtures.

For use in a vacuum manifold system (e.g. Ludger LV system) or centrifuge equipped with 96-well plate rotor.

Post-exoglycosidase clean-up:

For removal of exoglycosidase enzymes and other protein and peptide contaminants from unlabelled glycan mixtures. This prevents contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove proteins before mass spectrometry analysis of glycans.

Post-endoglycosidase Clean-up:

For removal of endoglycosidase enzymes and other protein and peptide material following enzymatic release of glycans from glycoproteins. Using this protein binding membrane will provide cleaner samples and increase the signal for the subsequent fluorescent labelling of the glycans. The plate can also be used to remove the enzymes and other protein and peptide material before permethylation and/or mass spectrometry analysis of glycans.