α-(1,6) core mannosidase

α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.  The presence of fucose linked to the core N-acetylglucosamine does not affect cleavage.

The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase (E-AM01), if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added after digestion by other mannosidases.

α(1-6) Core Mannosidase is useful for determining mannose linkages and rapidly removing the α(1-6) core mannose resistant to other mannosidases