α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.
α(1-3, 4) Fucosidase is useful for:
- Fucose linkage determination
- Deglycosylating glycoproteins with Lewis structures